Generator

Part:BBa_K3174002

Designed by: iGEM19_Austin_UTexas   Group: iGEM19_Austin_UTexas   (2019-08-08)
Revision as of 20:30, 8 August 2019 by Dbhat (Talk | contribs) (→‎Usage and Biology)


Blue Fluorescent Protein with Strong Promoter and Strong RBS

This part has the blue fluorescent protein (mTagBFP) gene inserted behind a strong promoter and strong ribosome binding site.


Usage and Biology

UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor (https://2019.igem.org/Team:Austin_UTexas
) and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
functionBurden: 17.8 +/- 1.6%