Coding
GlyRS

Part:BBa_K2916003

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-23)
Revision as of 10:48, 20 September 2019 by Konstantinos (Talk | contribs)


GlyRS protein equipped with a 6x HIS affinity tag

Glycyl-tRNA synthetase is a fusion protein that is used in the PURE and OnePot PURE cell-free systems.


Usage and Biology

Used in OnePot PURE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1737
    Illegal AgeI site found at 471
    Illegal AgeI site found at 2859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 958


[edit]
Categories
//cds/enzyme/ligase
Parameters
None