Coding
ArgRS

Part:BBa_K2916000:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-07-19)
Revision as of 14:53, 22 July 2019 by Konstantinos (Talk | contribs) (→‎Design Notes)


ArgRS protein equipped with a 10x HIS affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 76
    Illegal SapI.rc site found at 1495


Design Notes

The protein was cloned in the pQE30 vector and expressed in the M15 E.coli strain

Source

Escherichia coli gene alaS

References