Plasmid

Part:BBa_M50477

Designed by: Pedro Gallardo   Group: Stanford BIOE44 - S11   (2018-10-18)
Revision as of 01:19, 12 December 2018 by Pedro97 (Talk | contribs)

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Heat-shock-indued chromoprotein expression plasmid (pHeat)

This plasmid senses warm temperatures using the heat-shock protein 70 promoter (hsp70) and produces red pigment (meffRed) when activated. The warmer the temperature, the more red pigment that is produced. meffRed is tagged with a 6X-His tag, which facilitates Western blot analysis of meffRed production.

Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended.

Heat-shock-indued chromoprotein expression plasmid (pHeat)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 547
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