Regulatory

Part:BBa_K2624000:Experience

Designed by: Yunge Lou, Yali Wu   Group: iGEM18_CPU_CHINA   (2018-08-22)
Revision as of 03:33, 8 December 2018 by KKehuandiCPU (Talk | contribs) (→‎Applications of BBa_K2624000)

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Applications of BBa_K2624000

To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.

20181017205901%21T--CPU_CHINA--hp-qidongzi.png

Figure. The activity of the promoters

User Reviews

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