Part:BBa_K2876011

IL-1 Beta scFv2

This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody to a lambda repressor (BBa_K2876001) (https://parts.igem.org/Part:BBa_K2876001) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).

MIT MAHE 2020

Summary

Interleukin-1β (IL-1β), belongs to the interleukin-1 (IL-1) cytokine family. It is a pro-inflammatory cytokine and key in mediating the body's responses to microbial infection, immunological reactions, and tissue injury. Processing of IL-1β precursor to active form can be done using various proteases. It is involved in mediating inflammation, initiating or increasing a wide variety of non-structural, function-associated genes that are usually expressed during inflammation. There are now full cDNA sequences and genomic organization of IL-1 beta taken from bird, amphibian, bony fish, and cartilaginous fish, with many of these genes having been obtained using a homology cloning approach. This part produces a single-chain antibody that targets the immunoprotein interleukin B2.

References

Husain, M., Bird, S., van Zwieten, R., Secombes, C. J., & Wang, T. (2012). Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish. Developmental and comparative immunology, 38(3), 431–446. https://doi.org/10.1016/j.dci.2012.07.010

                                                                                                                                                                                   Bird, S., Zou, J., Wang, T., Munday, B., Cunningham, C., & Secombes, C. J. (2002). Evolution of interleukin-1beta. Cytokine & growth factor reviews, 13(6), 483–502. https://doi.org/10.1016/s1359-6101(02)00028-x 

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]