Coding

Part:BBa_K2876011

Designed by: Eleanor Glockner   Group: iGEM18_Stanford   (2018-10-10)
Revision as of 02:40, 18 October 2018 by Ijustice (Talk | contribs)


IL-1 Beta scFv2

This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody to a lambda repressor (BBa_K2876001) (https://parts.igem.org/Part:BBa_K2876001) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/immune_regulation/antibodies
Parameters
None