Coding
LeuABCD

Part:BBa_K2588015

Designed by: Dominika Wawrzyniak, Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-05)
Revision as of 00:42, 18 October 2018 by BKlopprogge (Talk | contribs)

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Leu Operon coding sequences from E. coli

We designed leuABCD to make E. coli produce 3-Methyl-1-butanole as a lure molecule to attract mosquitos. leuABCD is only one part of this lure module. leuABCD consists of four genes leuA, leuB, leuC and leuD. Its function is to catalyze the reaction of 2-ketoisovalerate to 2-ketoisocaproate.

Usage and Biology

leuABCD is a gene naturally occurring in the genome of E. coli. Its translation leads to the formation of four different proteins which are involved in the synthesis pathway of L-Leucine. LeuA converts 2-ketoisovalerate into 2-isopropylmalate. LeuB and LeuC then convert it into 3-isopropylmalate. LeuD converts it into 2-isopropyl-3-oxosuccinate and 2-isopropyl-3-oxosuccinate then spontaneously transforms into 2-ketoisocaproate,1 which is the substrate for the following pathway to form 3-Methyl-1-butanole.2

LeuABCD is part of the 3MB module, BBa_K2588024

References

  1. Connor, M.R., Cann, A.F. & Liao, J.C. Appl Microbiol Biotechnol (2010) 86: 1155.
  2. Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol, Michael R. Connor, James C. Liao, Appl. Environ. Microbiol. Sep 2008

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4340
    Illegal SapI.rc site found at 1172


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