Part:BBa_K2758000
Mercury activated Killer Red Protein suicide switch
The Killer Red Construct is a double condition suicide sequence. The first condition is the presence of mercury. When the bacteria are in an environment containing mercury, the production of RBS (BBa_B0030) is initiated through the Promotor (BBa_K346002). Then, the Killer Red protein (BBa_K1184000) is produced. The second condition is the presence of light. In order for the Killer red to activate, light (540-585 nm) needs to be shined onto the bacteria to initiate the reaction.
Usage and Biology
This part is designed to work in conjunction with a mercury accumulator device, specifically part BBa_K1355003 designed by the iGEM14_UFAM_BRAZIL team.
We chose to use BBa_B0030 as a moderate binding RBS in order to allow a delay in the expression of Killer Red protein in relation to the accumulator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 237
Illegal BsaI.rc site found at 528
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