Part:BBa_K2719004:Experience

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Applications of BBa_K2719004

This part was optimized Escherichia coli BL21 (DE3) so it could be transformed into it and also cloned in pSB1C3. For proving it presence it was necessary to make two agarose gels, one for the entire plasmid (figure 2) and the other one for the restriction part in order to observe the insert. (Figure 3)

Figure 1. Colonies transformed with Tenascin Fibronectin domains.

Figure 2. 0.85% agarose gel with GelRed, MW)NEB 1Kb Plus DNA Ladder, 1)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004), 2)Extraction Tenascin 5 Fibronectin domains (BBa_K2719004) duplicate.

Figure 3. 0.85% agarose gel with GelRed, MW)2-Log DNA Ladder, 8)Restriction Tenascin 5 Fibronectin domains with NotI (BBa_K2719004).

User Reviews

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