Coding

Part:BBa_K2684005

Designed by: Yihao Zhang   Group: iGEM18_SHSBNU_China   (2018-10-08)
Revision as of 21:31, 17 October 2018 by Snaaakskin (Talk | contribs)


SpyCatcher-CotA

SpyCatcher fused with CotA by 2xGGGGS linker

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 841


This part could be used to produce CotA laccase fused with SpyCatcher (BBa_K2684005). Team SHSBNU_China designed this part to improve the the enzyme activity of traditional CotA. When applying the gene CotA without SpyCatcher, the laccase cannot be suspended onto the biofilm created by CsgA-Spytag. Therefore, there will not be significant improvement in enzyme activity. However, while applying with CsgA-Spytag, the enzyme activity dramatically increased, as shown in the graph below.

The * notation stand for the significant of difference in the group, the more *a group has the more significant the difference in the group would be

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Categories
Parameters
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