Regulatory
Part:BBa_K2624000:Experience
Designed by: Yunge Lou, Yali Wu Group: iGEM18_CPU_CHINA (2018-08-22)
Revision as of 20:17, 17 October 2018 by JunQing Flag (Talk | contribs) (→Applications of BBa_K2624000)
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Applications of BBa_K2624000
To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter.
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