Part:BBa_R0040

TetR repressible promoter

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].

Usage and Biology

Medium strength promoter. [jb, 5/24/04]

From the reference article:

"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."

Improved by Fudan-CHINA in 2018

This year, Fudan-CHINA makes a mutation to Part:BBa_R0040 to improve its combination with the tTA transcriptional factor by computational simulation.Please be free to visit Part:BBa_K2886010.

Improved by University of Groningen in 2019

University of Groningen has improved the pTet promoter in E. voli using a synthetic promoter library on pTet (BBa_R0040) in order to enhance function and inducibility. Please visit Part:BBa_K3171173 for more info.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]