Coding
CcaS#3

Part:BBa_K2627001:Design

Designed by: Zhaoqin Zhang   Group: iGEM18_SDU-CHINA   (2018-09-30)
Revision as of 19:20, 17 October 2018 by Kaku (Talk | contribs)

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Advanced CcaS with PAS domain knocked out


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1400
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design notes

removing two PAS domains by Gibson Assembly in Freiburg BioBrick assembly standard.

Source

CcaS originated in Synechocystis PCC 6803

Reference

Nakajima, M., et al., Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal. Scientific Reports, 2016. 6(1).