Composite

Part:BBa_K2569031

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-04)
Revision as of 18:49, 17 October 2018 by DDY (Talk | contribs)


PdnaA -NicA2

Expressing this BioBrick in E. coli cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in E. coli can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.

<img src="T--SKLMT-China--nicotine-pathway.png"/>

Fig. Nicotine degradation pathway

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None