Composite

Part:BBa_K2717016:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)
Revision as of 18:48, 17 October 2018 by ChenyuLiu (Talk | contribs) (→‎Source)

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RBS2000-mCherry-protection coding2-GFP reverse-scar-RBS2000 reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1671


Design Notes

We adjusted the codon of the last lysine of GFP from AAA to AAG.

Source

GFP and mCherry is stored in our own lab.

References