Composite
Part:BBa_K2717009:Design
Designed by: Chenyu Liu Group: iGEM18_BNU-China (2018-10-09)
RBS2000-scar-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 677
Design Notes
We adjusted the codon of the last lysine of GFP from AAA to AAG.
Source
GFP and mCherry is stored in our own lab.