Composite

Part:BBa_K2557002

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-05)
Revision as of 17:55, 17 October 2018 by Chunfeng (Talk | contribs) (→‎Sequence and Features)


Bxb1 attB-RFP-Bxb1 attP

We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.


Usage and Biology

Sequence and Features

Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T undergone different experimental treatments. Transfection of different amounts of plasmids containing recombinase genes into cells.

Fig. 1 shows that this composite part works fine in the HEK 293T.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 837
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36
    Illegal BsaI site found at 177
    Illegal BsaI.rc site found at 885
    Illegal SapI site found at 794


Functional Parameters

[edit]
Categories
//chassis/eukaryote
Parameters
None