Part:BBa_K2623003

KaiC coding region

Summary

It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites(Fig3). Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state. A separate fragment is 1560bp.

Structure of KaiC

Fig.1 Planform of KaiC	Fig.2 Lateral view of KaiC	Fig.3 Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites

KaiC's phosphorylation stage

Fig.4 Simplified scheme of the central circadian oscillator system

More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 148
  Illegal AgeI site found at 859
  Illegal AgeI site found at 868
  Illegal AgeI site found at 874
  Illegal AgeI site found at 1198
  Illegal AgeI site found at 1225
  
• 1000
  COMPATIBLE WITH RFC[1000]