Plasmid backbones

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Welcome to the one-stop shop for all plasmids and plasmid related parts.

(This page is a work in progress. Thank your for your patience. If you'd like to contribute, contact [http://openwetware.org/wiki/User:Reshma_P._Shetty Reshma Shetty])

Help!

• Glossary - What are plasmids, vectors and backbones?

• Plasmid features - Common features of plasmids

• Plasmid naming - How BioBrick vectors are named

• Choosing the right plasmid - Choosing which plasmid to use

• Finding plasmids - How to locate plasmids in the Registry

• Constructing new plasmids - How to construct new BioBrick plasmids

Plasmids for use while assembling BioBrick parts

Are you trying to assemble two BioBrick parts together? There are a set of plasmids available designed to make assembly of BioBrick parts easier.

Plasmids for use while measuring or characterizing BioBrick parts

Are you interested in measuring the behavior of a BioBrick promoter, ribosome binding site, or transcriptional inverter? Jason Kelly has designed various plasmids to make the process of part characterization much easier.

Plasmids for use while operating or running BioBrick devices and systems

Oftentimes, it pays to use a different set of plasmids for operating or running your BioBrick device or system than you use for assembly. For example, while high copy plasmids are great for getting good DNA yields when doing plasmid purification, some BioBrick devices and systems consume too many resources when operated on a high copy plasmid. In those cases, you might want to switch to a low or medium copy plasmid.

Other plasmids that do not meet the BioBrick assembly standard but are still useful

Older plasmids that have been replaced by newer BioBrick plasmids