Coding
CAHS

Part:BBa_K2623008

Designed by: Weizhao Li   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 18:04, 17 October 2018 by Bb1984 (Talk | contribs) (→‎Summary)


CAHS coding region

Summary

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.

CAHS Fig1.png


CAHS_protein_Gel.png
As you can see, when the water bear is suffering slow drying, the TDPs will upregulate, and then he will become a crystal. Magically, after rehydration, he is still alive. We chose the CAHS1 which play a important role in this process.
A separate fragment is 714bp. More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 294
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None