Coding
Part:BBa_K2684002
Designed by: Yihao Zhang Group: iGEM18_SHSBNU_China (2018-10-08)
PhoA-CotA
CotA laccase attached with PhoA signal peptide sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1408
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 346
Usage and Biology
PhoA is a signal peptide attached to CotA coding sequence. PhoA signal peptide can help transfer CotA out of E.coli.
The enzyme activity was measured and calculated based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W.
For more experimental details, please visit http://2018.igem.org/Team:SHSBNU_China/Experiments
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Categories
Parameters
None |