Coding
GSDMD L290

Part:BBa_K2632005

Designed by: Zhujun Xia   Group: iGEM18_HZAU-China   (2018-09-15)
Revision as of 00:37, 18 October 2018 by FengQC (Talk | contribs)

L290D mutant of full length Gasdermin D

Pyroptosis is a form of lytic programmed cell death with inflammation. Recent studies reported that N-terminal of Gasdermin D (BBa K2632003) acts as an effector of pyroptosis1. Full length Gasdermin D is cleaved by Caspase 1 and then release the PFD (pore-forming domain) which can oligomerize on the cell membrane. Formation of pores causes cell to swell, leading to rupture of the membrane and massive leakage of cytosolic contents. L290D mutation of full length Gasdermin D reduces the cell toxicity.

Reference

1. Ding J, Wang K, Liu W, et al. Pore-forming activity and structural autoinhibition of the gasdermin family[J]. Nature, 2016, 535(7610):111.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883


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Categories
Parameters
None