Coding

Part:BBa_K2888000:Design

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-06)
Revision as of 16:52, 17 October 2018 by Yinchizhou18 (Talk | contribs) (→‎Design Notes)


cyanophage lysozyme gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We directly copy the sequence from the previous experiment by Kunal Mehta, Niklaus Evitt and James Swartz. However, since it has the unexpected restriction sites, we have replaced them with corresponding code.

Source

This cyanophage lysozyme is discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb} among 29 lysozyme family.

References

Jensen HB, Kleppe K: Effect of ionic strength, pH, amines and divalent cations on the lytic activity of T4 lysozyme. Eur J Biochem 1972, 28:116–122.

Kunal Mehta, Niklaus Evitt, James Swartz: Chemical Lysis of Cyanobacteria. Journal of Biological Engineer, 9/10/2015