Composite

Part:BBa_K2533002:Design

Designed by: Ziyang Xiao   Group: iGEM18_HUST-China   (2018-09-08)
Revision as of 14:31, 17 October 2018 by Xzy1012 (Talk | contribs) (→‎References)

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RBS-ldhDnARSdR-RBS-lldP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 780
    Illegal BglII site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1461
    Illegal AgeI site found at 1924
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 2521
    Illegal AgeI site found at 2533
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 764
    Illegal BsaI.rc site found at 1035


Design Notes

LdhDnARSdR is ldhD (lactate dehydrogenase gene) with D176A/I177R/F178S/N180R. LldP ​​protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhDnARSdR-lldp using molecular biology experiment methods.

Source

From Lactobacillus bulgaricus.

References

[1]Li, C., Tao, F., Ni, J., Wang, Y., Yao, F., & Xu, P. (2015). Enhancing the light-driven production of d-lactate by engineering cyanobacterium using a combinational strategy. Scientific Reports, 5, 9777. http://doi.org/10.1038/srep09777