Part:BBa_K2818002
Cas13b-NES-ADAR
dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required. It is an optimized construct obtained developed by Zhang Feng's lab (Feng et. al., 2017) to mediate efficient adenosine to inosine on specific positions on mRNA target, which can be programmed by a specific guide RNA. This construct is submitted as it is currently not in the iGEM registry and it acts as an important basis for comparison when we characterize our new part this year (<a href="https://parts.igem.org/Part:BBa_K2818001">BBa_K2818001</a>).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2884
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1787
Illegal BamHI site found at 839
Illegal BamHI site found at 3310
Illegal XhoI site found at 4011 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1520
Illegal AgeI site found at 2396 - 1000COMPATIBLE WITH RFC[1000]
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