Coding
Part:BBa_K2789024:Design
Designed by: Tianyi Chang Group: iGEM18_WHU-China (2018-10-06)
Revision as of 12:48, 17 October 2018 by QY2018WINNING (Talk | contribs)
Lectin-OmpA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 307
Illegal AgeI site found at 496
Illegal AgeI site found at 634 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is coding sequence of OmpA and lectin. You can add promoter and terminator to make it express the lectin on the surface of bacteria to bind with agents that have a lot of negative charges.
We synthesized the whole pathway in Pet28a, and use PCR to make this standard coding sequence.
Primer:
For :5’- CGGAATTCGCGGCCGCTTCTAGAG ATGAAAGCCACCAAACTGGTTCTGGG-3’
Rev:5’- GGTACTAGTAGCGGCCGCTGCAG TTATTACGGACCCGGACTGCTACCTGTG-3’
Source
We found it originally in genome of Green Algae.