Composite
NG6

Part:BBa_K2623026

Designed by: Ruofan Yang   Group: iGEM18_XMU-China   (2018-10-02)
Revision as of 12:32, 17 October 2018 by Hyydfl2 (Talk | contribs) (→‎Usage and Biology)

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Archaeal ribosomal protein protein L7Ae fused with SpyCatcher.

Summary

Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry. It's a non-RFP form of BBa_K2623025 and will be used to construct our total circuit.

Usage and Biology

To ensure which time point we should add the inducer IPTG, we measured the growth curve of BBa_K2623026 by detecting the OD 600 nm of E. coli(BL21) every hour.


Figure 1. The OD600 versus time of E.coli transfected with this part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1168
    Illegal NheI site found at 1191
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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