Tag

Part:BBa_K2719000:Design

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)
Revision as of 11:13, 17 October 2018 by AKARAM (Talk | contribs) (→‎Source)


GST


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

GST coding sequence is the one that is use for give structure and stability to Tenascin domain V. The binding is created by a polyproline linker which is going to maintain the original three dimensional structure of the tenascin domain V. GST has a high level of stability because of its structure and we can see it in figure 1. Its structure is mainly composed by alpha-helix structures giving it more stability to it structure. (Chakrabartty and Baldwin, 1995)


"T--TecCEM--GST3DStructure.png"

Figure 1. GST 3D structure , modelled with Swiss-Model

Source

This part was synthesized by IDT.

References

Chakrabartty, Baldwin. (1995). Stability of alpha-Helix. October 16, 2018, of Science Direct Website: https://www.sciencedirect.com/science/article/pii/S0065323308603344