Coding

Part:BBa_K2622003

Designed by: Simas Jasiūnas   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)
Revision as of 12:10, 17 October 2018 by GAMBIT (Talk | contribs)

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Immunoglobulin A-serine specific endopeptidase's translocator domain

Translocator domain from Neisseria gonorrhoeae (1087-1532 aa.) forms a β-barrel inside an outer membrane of Gram- bacteria. It has an Gly/Ser rich linker in N-terminus, which passes through the β-barrel to the outside of the bacteria. Fused protein particles to the N-terminus of the translocator domain will be displayed on the surface of Gram- bacteria. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None