Coding
Part:BBa_K2596005
Designed by: Matthew Mullin Group: iGEM18_Stony_Brook (2018-10-10)
cscB (sucrose permease) from E. coli
This is a sucrose symporter protein from E. coli.
Usage and Biology
CscB is a cell membrane protein that plays an integral role in transporting sucrose across the membrane as it is a sucrose-proton symporter. It is compatible in many strains of Synechococcus elongatus, including 2973, 6803 and 7942. S. elongatus is unique in that its cell membrane proton gradient is generated by maintaining a surplus of protons inside the cell. Sucrose can then be exported from the cell using the proton gradient.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 604 - 1000COMPATIBLE WITH RFC[1000]
Sucrose Assays
For experimental methods and results on characterization, look at the page for composite part BBa_K2596015.
[edit]
Categories
Parameters
//cds/membrane/pump
//chassis/prokaryote/cyanobacterium
//chassis/prokaryote/cyanobacterium
biology | Synechococcus elongatus PCC 7942 |
device_type | Coding sequence |