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Part:BBa_K2789023

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 08:16, 17 October 2018 by Talent b (Talk | contribs)


PPX+RBS

This part actually is a by-product during PCR but we found that this part can make a contribution to pathway establishment! the coding sequence PPX is originally from 15_York, and both two teams(15_York and 18_WHU) made a good verification of its function. Since it has a RBS just after its cds, you could build a pathway by just adding another coding sequence downstream this sequence.

We designed a primer to copy the PPX+RBS on the <a>BBa_K2789032</a>.

Application

This part can be added before a coding sequence to express it with the PPX.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 872


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Parameters
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