Reporter

Part:BBa_K2611001:Design

Designed by: Changhe Li   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 05:45, 17 October 2018 by White (Talk | contribs) (→‎References)

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spacer J23101-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 30
    Illegal NheI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


Design Notes

This part should be used with sgRNA(spacer J23101-GFP)(BBa_K2611000).


Source

The spacer sequence is selected from a reported sequence in which the function of this spacer has been proved. And the backbone we used is from BBa_J364000.

Referance:Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.

References

Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.