Composite

Part:BBa_K2812007:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brls   Group: iGEM18_TU-Eindhoven   (2018-10-08)
Revision as of 01:55, 17 October 2018 by GuidoOerlemans (Talk | contribs) (→‎Design Notes)


Coding sequence for Pyocin S5 with HlyA and His6-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal BglII site found at 425
    Illegal BglII site found at 2110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1200


Design Notes

Original DNA sequence based on Pseudomonas aeruginosa PA01 genome sequence, the nucleic acid sequences at positions 1067817–1066321 (the gene number: PA0985).1 This DNA sequence is codon optimized for E. coli. AraC promotor BBa_K2812002 was added to in front of this sequence.

Source

Genomic sequence from Pseudomonas aeruginosa. DNA construct produced by IDT.

References

1) Ling, Hua, et al. "A predicted S‐type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage." FEBS letters 584.15 (2010): 3354-3358.