Device
PcaU

Part:BBa_K2825002

Designed by: Chris Kuffner   Group: iGEM18_UMaryland   (2018-10-10)
Revision as of 01:27, 17 October 2018 by 15kuffnerc (Talk | contribs)


PcaU inducible promoter with high sensitivity

Inducible promoter for protocatechuic acid (PCA). The PcaU transcription factor represses its own transcription as well as a target transcript when PCA is not present by binding to the operator region. When PCA is present, PcaU releases the region and transcription of the target transcript occurs.


Usage and Biology

PcaU has been inserted into the pGLO plasmid, in which it expresses GFP when induced by PCA. Fluorescent intensity scales hyperbolically with PCA concentration. Above 10mM concentration, PCA was toxic to BL21 E. coli containing the PcaU reporter.

<img src="T--UMaryland--PCAUhigh.png">


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 171


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/regulatory/other
//function/regulation/transcriptional
//function/sensor
//promoter
//regulation/positive
Parameters
None