Composite

Part:BBa_K2558215:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 16:45, 17 October 2018 by MairZhou (Talk | contribs) (→‎Design Note)

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CRISPRi Safety Catch device with lux pR promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5547
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4314


Design Note

Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.

Source

References