DNA
FRT

Part:BBa_J72001:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-15)
Revision as of 05:11, 16 July 2008 by JCAnderson (Talk | contribs)

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{FRT} recombination site for flp recombinase in BBb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 18
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Overlap extension of ca1090F and ca1115R (71bp, EcoRI/BamHI)		
sub into pBca9145-Bca1089	         (EcoRI/BamHI, 2063+696, L)
Product is pBca9145-Bca1115
----
ca1090F	Forward EcoRI/BglII wobble for RBS part
gatttctggaattcatgAGATCT
ca1115R	FRT basic BBb part by wobble
cttgcggatccgaagttcctattctctagaaagtataggaacttcgcAGATCTcatgaattccagaa

References