Coding

Part:BBa_K209023

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)
Revision as of 19:36, 16 October 2018 by IndigoLi (Talk | contribs)


Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2018 Peking Team improvement

Team Peking 2018 improved this part.

First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope. https://parts.igem.org/Part:BBa_K2601008">BBa_K2601008

Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.

href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025 href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026 href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027

What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin. <a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a>

Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry. <a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a>
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a>

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