Coding

Part:BBa_K2729006

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-29)
Revision as of 18:14, 16 October 2018 by Crane (Talk | contribs)


DENV1 ancC-prM-E (Dengue Virus Serotype I)

Usage and Biology

EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP, and is codon-optimized for higher expression in mammalian cells. FMDV 2A is "self-cleaving" peptides, and upstream coding region can be a single cistron with the assistance.

Characterization

EGFP is derived from the jellyfish Aequorea victoria and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis


As shown in Figure 1, the band comes between 900 bp to 1,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for transfection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None