Coding

Part:BBa_K2680539:Design

Designed by: Stephanie Do   Group: iGEM18_William_and_Mary   (2018-10-15)
Revision as of 15:53, 16 October 2018 by Skdo (Talk | contribs) (→‎Source)

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TlpA39


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was taken from a publication from a lab at the California Institute of Technology.Forbidden restriction sites were checked for and removed if necessary. 1

Source

Organism: Salmonella typhimurium
TlpA is a "orthogonal, temperature-dependent transcriptional repressors providing switch-like control of bacterial gene expression 1 Original sequence: Piraner, Dan I., et al.

References

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.