Part:BBa_K2729013

DENV4 ancC-prM-E (Dengue Virus Serotype IV)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis

As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 488
  Illegal EcoRI site found at 1947
  Illegal SpeI site found at 1219
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 488
  Illegal EcoRI site found at 1947
  Illegal SpeI site found at 1219
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 488
  Illegal EcoRI site found at 1947
  Illegal BamHI site found at 1910
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 488
  Illegal EcoRI site found at 1947
  Illegal SpeI site found at 1219
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 488
  Illegal EcoRI site found at 1947
  Illegal SpeI site found at 1219
  Illegal NgoMIV site found at 1207
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 793
  

Reference

- Membranotropic regions of the dengue virus prM protein