Coding

Part:BBa_K2729004

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-29)
Revision as of 14:14, 16 October 2018 by Hajime Fujita (Talk | contribs)


DENV3 E (Envelope Protein | Dengue Virus Serotype III)

Usage and Biology

Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the qualitative experiment - description comes here.


As shown in Figure 1, the band of DENV3 E come around 1,500 bp.

Analysis

Our team successfully validated that the sequence of DENV3 E was synthesized, and obtained enough amount of it for the transduction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
    Illegal XhoI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250
    Illegal SapI.rc site found at 1078

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.

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