Signalling
Part:BBa_F1610
Designed by: Caitlin Conboy and Jennifer Braff Group: Antiquity (2003-12-04)
3OC6HSL Sender Device
This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
Notice
With a VF2-VR PCR length compraison, I found that it is empty on this plasmid sent in iGEM2007 plates. --marion 07:12, 22 March 2008 (EDT)
While present on a gel after running PCR on both 2007 and 2008 plates, the DNA appears to only be ~600bp long which conflicts with the part description. --robere 27 Jun 2008
See gel image
We use this part on iGEM2009 plate. While present on a gel after cutting the miniprep DNA extraction by EcoRI and SpeI, the restriction fragment appears to about 3000 bp and 800 bp . BBa_F1610 on iGEM2009 plate is therefore correct.--Koi 20 Oct 2009 (Tokyo_Tech)
See gel|image
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 655
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/signalling/sender
//function/cellsignalling
//function/cellsignalling
control | |
device_type | Sender |
family | |
latency | |
output | |
proteins | LuxI |
signalling_molecule | 3OC6HSL |