Coding

Part:BBa_K2686002:Design

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-09-30)
Revision as of 12:40, 16 October 2018 by Caffeine4Life (Talk | contribs) (→‎Design Notes)


Encapsulin protein with HexaHistidine insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457


Design Notes

The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was codon optimized for bacterial expression in E. coli and introduced in between amino acids 43/44 in .

Source

Derived from BBa_K2686001.

References

Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801.