Part:BBa_K1796007
nifB from Paenibacillus sp. WLY78
essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1156
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1156
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1156
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1156
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1156
Illegal AgeI site found at 129
Illegal AgeI site found at 1089
Illegal AgeI site found at 1444 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1261
Parameter of Protein
Number of amino acids: 499
Molecular weight: 54869.8
Theoretical pI: 6.64
Amino acid composition:
Ala (A) 46 9.2%
Arg (R) 35 7.0%
Asn (N) 17 3.4%
Asp (D) 23 4.6%
Cys (C) 16 3.2%
Gln (Q) 19 3.8%
Glu (E) 40 8.0%
Gly (G) 42 8.4%
His (H) 17 3.4%
Ile (I) 30 6.0%
Leu (L) 41 8.2%
Lys (K) 25 5.0%
Met (M) 11 2.2%
Phe (F) 13 2.6%
Pro (P) 25 5.0%
Ser (S) 27 5.4%
Thr (T) 17 3.4%
Trp (W) 2 0.4%
Tyr (Y) 13 2.6%
Val (V) 40 8.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:Carbon C 2398
Hydrogen H 3849
Nitrogen N 701
Oxygen O 719
Sulfur S 27
Formula: C2398H3849N701O719S27Total number of atoms: 7694
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 31370
Abs 0.1% (=1 g/l) 0.572, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.553, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitr
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