Regulatory

Part:BBa_K2596001

Designed by: Priya Aggarwal, Jennifer Rakhimov   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 04:16, 16 October 2018 by Kledalla (Talk | contribs) (→‎Usage and Biology)


PcpcB from Synechococcus elongatus PCC7942

PcpcB is a constitutive promoter from cyanobacteria Synechococcus elongatus PCC 7942.

Usage and Biology

cpcB codes for phycocyanin, a phycobiliprotein complex, and works to harvest light energy before transmitting it to the chlorophylls. The promoter is a strong promoter but also demonstrates expression patterns following circadian oscillations. Studies have found that Pcpc can also be used in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

PcpcB1A1 features two promoter sequences within the promoter, both of which were included in the BioBrick. For this promoter, derived from Synechococcus elongatus PCC 7942, we kept about 100 bp of upstream and downstream regions, the latter of which was cropped immediately before the ATG start codon. Studies have found that Pcpc can also be used in E. coli.


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//promoter
Parameters
biologySynechococcus elongatus PCC 7942
device_typeConstitutive Promoter