Coding
Part:BBa_K2764000
Designed by: CHU Ora-In Group: iGEM18_Hong_Kong_HKUST (2018-10-03)
Alkane channel protein
The alkL protein is an alkane channel protein that comes from Pseudomonas Oleovorans. Its function is mainly to import medium to long chain alkanes into the cell. In Gram-negative cells, such as Escherichia coli and Shewanella oneidensis, import of medium to long chain alkanes is inhibited by the cell's lippopolysaccharide layer. The alkL protein's extracellular domain penetrates this layer and binds to alkane molecules with high affinity. Once the alkane has been bound, it is transported through the hydrophobic core of the alkL protein, thus bypassing the lipopolysaccharide layer, and enters the periplasm of the cell, where it can then diffuse into the cytoplasm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 651
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 303
Illegal NgoMIV site found at 516 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |