Part:BBa_K2729010

DENV1 ancC-prM-E (Dengue Virus Serotype I)

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1831
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1831
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1245
  Illegal BglII site found at 1856
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1831
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1831
  Illegal AgeI site found at 1450
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 217
  Illegal BsaI.rc site found at 367
  

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

reference

- [https://pubs.acs.org/doi/10.1021/bi500724k Nemésio H, Villalaín J. Membranotropic regions of the dengue virus prM protein. Biochemistry. 2014 Aug 19;53(32):5280-9. doi: 10.1021/bi500724k. Epub 2014 Aug 6. PubMed PMID: 25076193. ]