Part:BBa_K2637037

Gal1 promoter+EYFP+ADH1T

We constructed this pRS416 plasmid carrying the genes of EYFP with the Gal1 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the EYFP fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1125