Regulatory

Part:BBa_K091107:Experience

Designed by: Xiao Zhu   Group: iGEM08_Davidson-Missouri_Western   (2008-06-19)
Revision as of 01:39, 28 October 2010 by Ajk5319 (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K091107

User Reviews

UNIQ6743c19c2be76dcf-partinfo-00000000-QINU UNIQ6743c19c2be76dcf-partinfo-00000001-QINU

Andrew Kirk, undergraduate, Penn State iGEM 2010

Two Lux-inducible promoters that were already on the registry (K091107 and K091146) were characterized. Because these promoters require AHL and LuxR in order to activate, a construct was created that consisted of a Lux promoter followed by RFP, and a constitutive promoter followed by the LuxR gene. LuxR was expected to be present in excess so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034.

The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM.

The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki]show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters.